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The human genome is the total set of genes carried by an individual. Genomics — the scientific study of the genome — plays a major role in the Inflammation and the Host Response to Injury program because of the significant influence that genetic factors have on human characteristics, including the body’s response to injury and burns. Functional genomics refers to the use of experimental approaches to study gene function systematically (genome-wide). These tools include DNA microarrays, “chips” that can measure and compare relative levels of mRNA abundance in cells or tissues of interest.
By analogy with the genome, the proteome is the set of all proteins found in an individual. Tens of thousands of genes control the ultimate production of even more different types of proteins, including many that are involved in the human inflammatory response to injury.
In this program, our Proteomics Core groups use techniques such as high-throughput mass spectrometry and flow cytometry to explore the relationship between proteomics and the human response to injury.
Cell Separation and Sample Preparation
The overall goal of the Cell Separation and Sample Preparation (CSSP) Core is to develop technologies for the collection and isolation of enriched blood leukocyte cell populations that are applicable to critically-ill patient populations for subsequent high-throughput proteomic and genomic analyses, as well as for functional proteomics. The Core also assists with the collection, processing and archival of solid tissue samples obtained at surgical interventions in the trauma and burn patient populations.
The Core has several responsibilities within the Program:
Because of these distinct functions, the Core has both development and service components similar to the Genomics Core.